Dr. Raphael Pavani
National Cancer Institute, NIH
Dr. Raphael Pavani is a molecular biologist passionate about unraveling the intricacies of genome integrity and has dedicated his career to advancing our understanding of DNA replication, repair, and the molecular processes underlying diseases. Dr. Pavani earned his master’s and Ph.D. degrees from the University of Sao Paulo in Brazil, where he studied DNA replication, repair, and cell cycle control in the pathogenic protozoan parasites T. cruzi and T. brucei, causative agents of Chagas Disease and Sleeping Sickness, respectively. During his Ph.D. studies, Dr. Pavani also embarked on an enriching internship at the University of Dundee in the UK, further broadening his expertise in biological chemistry and drug discovery. His PhD work resulted in numerous publications and the prestigious “Outstanding PhD Thesis Award” from the University of Sao Paulo.
In 2019, Dr. Pavani joined the Laboratory of Genome Integrity at the National Cancer Institute (NCI) within the National Institutes of Health (NIH) in the United States, under the mentorship of Dr. André Nussenzweig. Among his significant contributions, he uncovered that the loss of EXO1 is synthetic lethal with BRCA1 deficiency, providing a promising avenue for targeted cancer therapies. Additionally, he developed a cutting-edge system to induce collapsed replication forks at specific genomic loci, revealing fundamental insights into replication-associated DNA damage. His research, recently published in Science, challenged the prevailing view that replication-associated breaks are exclusively one-ended breaks and demonstrated that homologous recombination (HR) is the primary repair pathway at broken forks. Moreover, he revealed that the mechanisms involved in repairing these lesions differ from those used in the repair of canonical, replication-independent double-strand breaks, advancing our understanding of DNA repair pathways and their therapeutic implications.